HELIXPrep › Plant, Animal and Microbial Biotechnology › Animals
Module 5.2 · Topic 7
Module 5.2 · Topic 7
RNA interference could silence a gene temporarily. This does it permanently.
RNA interference, several modules back, let researchers silence a specific gene in cell culture, temporarily, at the RNA level. This lesson's technique does something related but genuinely more permanent — disabling a gene at the DNA level itself, in an entire living animal, from birth.
RNAi silences a gene's mRNA transcript, but leaves the underlying DNA sequence completely untouched — the effect fades once the siRNA is degraded. A knock-out animal has the actual gene disrupted at the genomic level. Which effect is permanent?
A knock-out animal is created by:
Create a free account to track your progress, earn XP, and unlock every free module on HELIXPrep.
Create Free Account →