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Module 6.1 · Topic 4
Module 6.1 · Topic 4
Successfully delivered, and doing absolutely nothing
A gene of interest, cloned into a standard vector, replicates faithfully through every cell division — and produces essentially no protein at all. The vector did its job perfectly. Its job was never to make the gene express.
A gene is successfully cloned into a basic vector with an origin of replication and a selectable marker, but no promoter or ribosome binding site. Will the gene be expressed?
A gene cloned into a basic vector lacking a promoter and ribosome binding site will typically:
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