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Module 6.1 · Topic 6
Module 6.1 · Topic 6
One gene, among a million clones
A genomic or cDNA library exists as thousands of separate bacterial colonies, each carrying a different DNA fragment. The one gene actually needed could be any single one of them — and there's no way to tell just by looking.
A short DNA sequence, known to be complementary to part of the gene of interest, is labelled and used to search a library. What would this labelled probe do when it encounters the correct clone?
In hybridisation-based library screening, a labelled probe identifies the target clone by:
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