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Module 6.1 · Topic 7
Module 6.1 · Topic 7
More expression isn't always better
A gene expressed at extremely high levels in E. coli should, in principle, yield abundant protein. Instead, the cell often produces a dense, useless, insoluble clump — the protein technically made, but not remotely usable.
A cell's protein-folding machinery (chaperones) can only fold so much protein per unit time. If expression is pushed far beyond that capacity, what's the likely outcome?
Inclusion bodies form when:
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