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Module 6.1 · Topic 5
Module 6.1 · Topic 5
Two ways to capture 'everything the genome might contain'
A genomic library and a cDNA library, made from the exact same cell at the exact same moment, don't contain the same information at all. One captures the entire genome. The other captures only what that cell happened to be actively transcribing.
Back in Molecular Biology, RNA splicing showed that the initial transcript isn't the final message — introns get removed before translation. A cDNA library is built from mature mRNA, using reverse transcriptase. Would a cDNA library contain intron sequences?
A genomic DNA library, compared to a cDNA library from the same organism, contains:
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